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with adenine, converting a GC base pair to a TA base pair a transversion. (After C. Mol, et al., DNA repair mechanisms for the

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Since the collector current is controlled by the base current, the controlled-source symbol is used to represent the collector current Finally, when the base current becomes suf ciently large, the collector-emitter voltage, VCE , reaches its saturation limit, and the collector current is no longer proportional to the base current; this is called the saturation region The three conditions are described in Figure 922 in terms of simple circuit models The corresponding collector curves are shown in Figure 923

Properties of some hard and brittle materials [7] [11] [13]

50 45 40 35 30 25 20 15 10 5 0

recognition and removal of damaged DNA bases, Annual Review of Biophysics and Biomolecular Structure, 28:101 28,1999, gure 6.)

Example 97 illustrates the application of this large-signal model in a practical circuit and illustrates how to determine which of the three states is applicable, using relatively simple analysis

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The McGraw Hill Companies, 2001

Using Device Data Sheets One of the most important design tools available to engineers is the device data sheet In this box we illustrate the use of a device data sheet for the 2N3904 bipolar transistor This is an npn general-purpose ampli er transistor Excerpts from the data sheet are shown below, with some words of explanation The complete data sheet can be found in the accompanying CD-ROM

2N3904 NPN General Purpose Amplifier This device is designed as a general purpose amplifier and switch The useful dynamic range extends to 100 mA as a switch and to 100 MHz as an amplifier

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Transitions are routinely produced by base analogues. Two of the most widely used base analogues are the pyrimidine analogue 5-bromouracil (5BU) and the purine analogue 2-aminopurine (2AP; g. 12.20). The mutagenic mechanisms of the two are similar. The 5-bromouracil is incorporated into DNA in place of thymine; it acts just like thymine in DNA replication and, since it doesn t alter the hydrogen bonding, should induce no mutation. However, it seems that the bromine atom causes 5-bromouracil to tautomerize more readily than thymine does. Thus, 5-bromouracil goes from the keto form ( g. 12.20) to the enol form more readily than thymine. Transitions frequently result when the enol form of 5-bromouracil pairs with guanine.

Melting point ( C) 1371 1532 350 750 2040 2050 2830 2500 3200 3700

TO-92

ELECTRICAL CHARACTERISTICS:

The 2-aminopurine is mutagenic by virtue of the fact that it can, like adenine, form two hydrogen bonds with thymine. When in the rare state, it can pair with cytosine ( g. 12.21). Thus, at times it replaces adenine, and at other times guanine. It promotes transition mutations. Nitrous acid (HNO2) also readily produces transitions by replacing amino groups on nucleotides with keto groups ( NH2 to O). The result is that cytosine is converted to uracil, adenine to hypoxanthine, and guanine to xanthine. As gure 12.22 shows, transition

The section on electrical characteristics summarizes some of the important voltage and current speci cations of the transistor For example, you will nd breakdown voltages (not to be exceeded), and cutoff currents In this section you also nd important modeling information, related to the large-signal model described in this chapter The large-signal current gain of the transistor, hF E , or , is given as a function of collector current Note that this parameter varies signi cantly (from 30 to 100) as the DC collector current varies Also important are the CE and BE junction saturation voltages (the batteries in the large-signal model of Figure 922)

(5BU) and 2-aminopurine (2AP).

OFF CHARACTERISTICS V(BR)CEO V(BR)CBO V(BR)EBO IBL ICEX Collector-Emitter Breakdown Voltage Collector-Base Breakdown Voltage Emitter-Base Breakdown Voltage Base Cutoff Current Collector Cutoff Current IC = 10 mA, IB = 0 IC = 10 A, IE = 0 IE = 10 A, IC = 0 VCE = 30 V, VEB = 0 VCE = 30 V, VEB = 0 40 60 60 50 50 V V V nA nA

Thermal conductivity (W/m K) 15 52 06 17 29

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